Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 266-268, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334232
ABSTRACT
<p><b>OBJECTIVE</b>To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.</p><p><b>METHODS</b>By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.</p><p><b>CONCLUSIONS</b>HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Enzyme-Linked Immunosorbent Assay
/
Viral Vaccines
/
Chemistry
/
Amino Acid Sequence
/
Hepatitis C
/
Viral Nonstructural Proteins
/
Epitope Mapping
/
Peptide Library
/
Allergy and Immunology
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
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