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Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 266-268, 2002.
Article in Chinese | WPRIM | ID: wpr-334232
ABSTRACT
<p><b>OBJECTIVE</b>To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.</p><p><b>METHODS</b>By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.</p><p><b>CONCLUSIONS</b>HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / Enzyme-Linked Immunosorbent Assay / Viral Vaccines / Chemistry / Amino Acid Sequence / Hepatitis C / Viral Nonstructural Proteins / Epitope Mapping / Peptide Library / Allergy and Immunology Language: Chinese Journal: Chinese Journal of Hepatology Year: 2002 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / Enzyme-Linked Immunosorbent Assay / Viral Vaccines / Chemistry / Amino Acid Sequence / Hepatitis C / Viral Nonstructural Proteins / Epitope Mapping / Peptide Library / Allergy and Immunology Language: Chinese Journal: Chinese Journal of Hepatology Year: 2002 Type: Article