Transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 248-252, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334237
ABSTRACT
<p><b>OBJECTIVE</b>To investigate transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells.</p><p><b>METHODS</b>Enkaryotic expression plasmids inserted HBsAg gene or chimeric HBsAg-HSP70 gene were prepared and transfected into HepG2 cells by means of cationic liposome. mRNA were detected by RT-PCR and proteins expressed in the cells were detected by immunocytochemistry 48 hours later. HBsAg in cultured supernatants and cell lysates were assayed by ELISA.</p><p><b>RESULTS</b>Fusion protein (HBsAg-HSP70) transient expression in HepG2 cells were confirmed by RT-PCR, immunocytochemistry or ELISA, but fusion protein was not assayed in cell cultured supernatants by ELISA.</p><p><b>CONCLUSIONS</b>Transfection of HepG2 cells with a chimeric HBsAg-HSP70 construct leads to express fusion protein, but it does not secrete into cell cultured supernatants.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Bacterial Proteins
/
Recombinant Fusion Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
/
Hepatitis B Vaccines
/
HSP70 Heat-Shock Proteins
/
Vaccines, DNA
/
Reverse Transcriptase Polymerase Chain Reaction
/
Allergy and Immunology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
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