Effect of IL-12 on IFN-gamma and IL-10 produced by peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection during IFN-alpha treatment / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 116-119, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334296
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of IL-12 on IFN-gamma and IL-10 production of peripheral blood mononuclear cells (PBMC) in chronic hepatitis B virus infection patients during IFN-alpha treatment.</p><p><b>METHODS</b>Before and after IFN-alpha treatment of 3 months and 6 months, PBMC of 20 patients with chronic hepatitis B virus infection were collected and cultured in vitro in the culture fluid containing PHA (100 microg/ml), HBcAg (1 microg/ml), or HBeAg (1 microg/ml) for 48 h under the condition of the presence or absence of IL-12 (10 ng/ml). Then the levels of IFN-gamma and IL-10 were determined by ELISA.</p><p><b>RESULTS</b>There were 12 responders and 8 nonresponders to IFN-alpha treatment. In the responders, the enhancing effect of IL-12 on IFN-gamma production was significantly greater after IFN-alpha treatment than before the treatment. The production of IL-10 was suppressed in the presence of IL-12 after 3 months and 6 months of IFN-alpha treatment. In the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12. To the nonresponders, the enhancing effect of IL-12 on IFN-gamma production was also significantly increased after IFN-alpha treatment. Moreover, in the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12.</p><p><b>CONCLUSIONS</b>The enhancing effect of IL-12 on IFN-gamma production of PBMC in patients with chronic hepatitis B virus infection is increased during IFN-alpha treatment. IFN-alpha and IL-12 may enhance the efficacy for the treatment of chronic hepatitis B virus infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pharmacology
/
Time Factors
/
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Leukocytes, Mononuclear
/
Interferon-gamma
/
Interleukin-10
/
Interferon-alpha
/
Interleukin-12
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
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