Expression of integrin alpha 6 in hepatic sinusoidal capillaration / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 90-92, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334306
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration.</p><p><b>METHODS</b>The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting.</p><p><b>RESULTS</b>We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05).</p><p><b>CONCLUSIONS</b>During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Time Factors
/
Carbon Tetrachloride
/
Immunohistochemistry
/
Immunoblotting
/
Antigens, CD
/
Laminin
/
Rats, Wistar
/
Integrin alpha6
/
Liver
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
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