Study of encapsulation of suicide gene in poly(D,L-lactic-co-glycolic acid) nanoparticles and its characteristics in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 30-33, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334334
ABSTRACT
We constructed the AFP promotor, suicide gene and EGFP eukaryotic expression vector recombinant plasmid, and this plasmid DNA was encapsulated by biodegradable, biocompatible polymer PLGA to prepare nanoparticles. The results demonstrate that the mean diameter of DNA-PLGA-NP is 68 nm, the encapsulation ratio reaches to 80%, and the PLGA nanoparticles can protect plasmid DNA from digestion by deoxyribonuclease I (DNaseI) and sonication-induced shearing in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Size
/
Plasmids
/
Polyglycolic Acid
/
Polymers
/
Recombination, Genetic
/
Biocompatible Materials
/
Biodegradation, Environmental
/
In Vitro Techniques
/
DNA
/
Lactic Acid
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2002
Type:
Article
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