Effects of blocking the CXC chemokine receptor 3 pathway on acute rejection of islet allograft / 中华外科杂志
Chinese Journal of Surgery
;
(12): 210-213, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-334374
ABSTRACT
<p><b>OBJECTIVE</b>To identify the effect of PNA CXCR3 on acute rejection of islet allograft.</p><p><b>METHODS</b>The mice islet transplant models were used. The mice were divided into three groups including saline group, PNA CXCR3 group and mismatch PNA group. In vitro the proliferation capability of T cell was assessed by proliferative responses. RT-PCR and western blot were used to detect the expression of mRNA and protein. Flow cytometry was applied to determine the expression level of CXCR3 in spleen CD3(+) T cells.</p><p><b>RESULTS</b>Compared with saline [(6.72 +/- 1.48) d] and PNA mismatch-treated recipients [(6.54 +/- 0.86) d], PNA CXCR3-treated recipients demonstrated statistically significant prolongation [(9.70 +/- 1.57) d] in functional allograft survival. The CXCR3 mRNA expression level of PNA CXCR3 group (1.06 +/- 0.07) was significantly down-regulated compared with saline (1.98 +/- 0.22) and PNA mismatch (1.87 +/- 0.10) group at the 7th day after transplant. The date showed that CXCR3 protein and lymphocytes proliferation capability was significantly down-regulated in PNA CXCR3 group compared with saline and PNA mismatch group (P<0.01).</p><p><b>CONCLUSIONS</b>The present study indicates that PNA CXCR3 can inhibit T cell activating and prolonging the survival time of islet allograft and has a substantial therapeutic effect on inhibiting acute allograft rejection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
General Surgery
/
Transplantation, Homologous
/
Signal Transduction
/
Random Allocation
/
Blotting, Western
/
Oligonucleotides, Antisense
/
Pancreas Transplantation
/
Peptide Nucleic Acids
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2007
Type:
Article
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