The regulation effect of interleukin-1 on expression of cartilage specificity gene Sox9 mRNA in the human intervertebral discs / 中华外科杂志
Chinese Journal of Surgery
; (12): 1704-1707, 2006.
Article
in Zh
| WPRIM
| ID: wpr-334425
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To assess the regulating effects of interleukin-1 (IL-1) on gene expression of cartilage specificity gene Sox9 and type II collagen mRNA in the human intervertebral discs.</p><p><b>METHODS</b>RT-PCR were used to investigate the effects of IL-1 on gene expression of Sox9 and type II collagen mRNA in intervertebral discs cells cultures of embryo.</p><p><b>RESULTS</b>The Sox9 and type II collagen mRNA in intervertebral discs were decreased progressively along with the addition concentrations of IL-1 than the controls. And the mRNA of Sox9 and type II collagen also markedly decreased with the time of culture.</p><p><b>CONCLUSIONS</b>IL-1 could cause dose-dependent and time-dependent inhibition effects on Sox9 and type II collagen gene expression in human intervertebral discs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Time Factors
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Transcription Factors
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RNA, Messenger
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High Mobility Group Proteins
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Gene Expression
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Cells, Cultured
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Interleukin-1
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Reverse Transcriptase Polymerase Chain Reaction
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Cell Biology
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Surgery
Year:
2006
Type:
Article