The regulation effect of interleukin-1 on expression of cartilage specificity gene Sox9 mRNA in the human intervertebral discs / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To assess the regulating effects of interleukin-1 (IL-1) on gene expression of cartilage specificity gene Sox9 and type II collagen mRNA in the human intervertebral discs.</p><p><b>METHODS</b>RT-PCR were used to investigate the effects of IL-1 on gene expression of Sox9 and type II collagen mRNA in intervertebral discs cells cultures of embryo.</p><p><b>RESULTS</b>The Sox9 and type II collagen mRNA in intervertebral discs were decreased progressively along with the addition concentrations of IL-1 than the controls. And the mRNA of Sox9 and type II collagen also markedly decreased with the time of culture.</p><p><b>CONCLUSIONS</b>IL-1 could cause dose-dependent and time-dependent inhibition effects on Sox9 and type II collagen gene expression in human intervertebral discs.</p>