Gene therapy targeting for carcinoma regulated by E2F-1 promoter / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1636-1639, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-334440
ABSTRACT
<p><b>OBJECTIVE</b>To study the selective effect to tumor cells mediated by a recombinant adenoviral vector carrying E2F-1 promoter.</p><p><b>METHODS</b>The AdEasy-1 adenoviral vector system was used in this experiment. Several recombinant adenovirus with tumor-targeting E2F-1 promoter were constructed and then the E2F-1 promoter gene was checked by PCR and sequencing. The two adenovirus expressing GFP gene which is regulated by E2F-1 promoter or CMV promoter were used to respectively transfect tumor cells and non-proliferating normal cells, then observed and analyzed the different results caused by different promoters. Vpr gene was cloned into the targeting recombinant adenovirus. The new adenovirus named rvAdE2F-1/vpr was used to transfect tumor cells SMMC-7721, LS174T and non-proliferating normal cells H292, L-02. The surviving rate of each group was registered; the level of E2F-1 protein expressed in normal and tumor cell lines were checked by Western Blot.</p><p><b>RESULT</b>E2F-1 promoter can regulate the downstream gene GFP selectively expressed in LS174T and its activity in LS174T was similar with CMV promoter's; Vpr gene regulated by E2F-1 promoter can suppress the proliferation of tumor cells and no toxicity to normal cells; In all of the tumor cells, a much higher level of E2F-1 was expressed compared with normal cell lines. E2F-1 promoter's activity correlated well with E2F-1 protein levels.</p><p><b>CONCLUSIONS</b>E2F-1 promoter can control a selective cell killing to cancer cells, with no effect to normal cells. The system of E2F-1 promoter is a useful method for tumor-targeting gene therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Therapeutics
/
Recombinant Fusion Proteins
/
Transfection
/
Genetic Therapy
/
Cell Line
/
Adenoviridae
/
Blotting, Western
/
Promoter Regions, Genetic
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2006
Type:
Article
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