P38 MAPK was involved in angiotensin-induced migratory potential of adventitial fibroblasts from spontaneously hypertensive rat / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 557-560, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-334657
ABSTRACT
<p><b>OBJECTIVE</b>To test whether P38 MAPK is involved in angiotensin II (Ang II)-enhanced migration potential of adventitial fibroblasts (AFs) from spontaneously hypertensive rat (SHR).</p><p><b>METHODS</b>Migratory potential was estimated by transwell chamber in vitro. Activation of P38 MAPK pathway was determined with phosphospecific antibodies by immunoblotting.</p><p><b>RESULTS</b>Ang II induced migration of SHR-AFs was markedly increased in a dose-dependent manner when compared with WKY-AFs. Addition of the Ang II receptor type-1 (AT1-R) antagonist Losartan and P38 MAPK inhibitor SB202190 suppressed Ang II-induced migration of SHR-AFs. Ang II could induce P38 MAPK phosphorylation in SHR-AFs in a time-and dose-dependent manner. Phosphorylation of P38 MAPK was suppressed by Losartan and SB202190.</p><p><b>CONCLUSION</b>This study indicated that Ang II-induced migration involves P38 MAPK pathway via AT1 receptor in aortic adventitial fibroblasts from SHR.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Rats, Inbred SHR
/
Rats, Inbred WKY
/
Angiotensin II
/
Signal Transduction
/
Cell Movement
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Cells, Cultured
/
Cell Biology
/
P38 Mitogen-Activated Protein Kinases
/
Fibroblasts
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2005
Type:
Article
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