Construction of mammalian cell lines continuously expressing JEV structural proteins / 病毒学报
Chinese Journal of Virology
;
(6): 279-285, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-334723
ABSTRACT
Based on the infectious clone of JEV vaccine strain SA14-14-2, prM-E genes and C-prM-E genes were cloned into pCDNA3.1 vector. The recombinant plasmid pCJE-ME was transfected into BHK-21 cells, the expressed proteins were toxic to the cell growth and accelerated the cell death. But when transfected with the plasmid pCJE-CME, the cell lines continuously expressing structural proteins could be selected with G418. And the expression products of pCJE-CME vector could be detected by ELISA, Western Blot and IFA assay. It showed that the JEV capsid protein could enhance the stability of the cell lines expressing the structural proteins. The established cell lines can make the acquirement of the virus-like particles much easier.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Gene Expression
/
Viral Structural Proteins
/
Cell Line
/
Cricetulus
/
CHO Cells
/
Apoptosis
/
Cell Biology
/
Capsid Proteins
/
Encephalitis Virus, Japanese
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2009
Type:
Article
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