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The expression and anti-apoptotic function of HCMV IE2 protein controlled by Tet-On system / 病毒学报
Chinese Journal of Virology ; (6): 190-195, 2009.
Article in Chinese | WPRIM | ID: wpr-334752
ABSTRACT
During the infection of host cells, IE2 protein is one of the first and most abundantly expressed products of HCMV genome, which plays an important role in the controlling of cell cycle and apoptosis. But the correlation between expression level and anti-apoptotic activity of IE2 protein is still not clear. In this study, we successfully established a HCMV IE2 protein expression cell line that was controlled by Tet-On system. The effect of IE2 protein on cell apoptosis and the expression of p53 was detected under different condition of induction. Our results showed that the IE2 protein could inhibit cell apoptosis induced by TNF-alpha. Additionally, the anti-apoptotic activity of IE2 protein seemed to be relevant to its expression level. However, we failed to detect any difference of p53 expression between the IE2 protein expression and non-expression cells. These data indicated that the IE2 protein might inhibit cell apoptosis through regulating different signal pathways.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plasmids / HeLa Cells / Trans-Activators / Gene Expression Regulation / Tumor Suppressor Protein p53 / Apoptosis / Doxycycline / Immediate-Early Proteins / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Virology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plasmids / HeLa Cells / Trans-Activators / Gene Expression Regulation / Tumor Suppressor Protein p53 / Apoptosis / Doxycycline / Immediate-Early Proteins / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Virology Year: 2009 Type: Article