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The first report of Kadipiro virus isolation in China / 病毒学报
Chinese Journal of Virology ; (6): 173-177, 2009.
Article in Chinese | WPRIM | ID: wpr-334755
ABSTRACT
5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Virology / Molecular Sequence Data / Cell Line / China / Amino Acid Sequence / Classification / Sequence Analysis, DNA / Coltivirus / Reverse Transcriptase Polymerase Chain Reaction Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Virology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Virology / Molecular Sequence Data / Cell Line / China / Amino Acid Sequence / Classification / Sequence Analysis, DNA / Coltivirus / Reverse Transcriptase Polymerase Chain Reaction Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Virology Year: 2009 Type: Article