Construction and analysis of full-length cDNA clone of rabies virus street strain / 病毒学报
Chinese Journal of Virology
;
(6): 17-22, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-334769
ABSTRACT
To construct a expression plasmid containing the full-length cDNA of rabies virus, four overlapped fragments covering full length cDNA of rabies virus street stain HN10 were cloned into pVAX1 sequentially in the genome except for the G-L noncoding region which was replaced with GFP gene. The plasmid containing the full-length viral cDNA was flanked by hammerhead ribozyme (HamRz) and hepatitis delta ribozyme (HdvRz) sequences and arranged under the control of the cytomegalovirus (CMV) promoter. The constructed plasmid could be directly used for the following procedure of producing the recombinant rabies virus street HN10.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Rabies virus
/
Classification
/
Cloning, Molecular
/
DNA, Complementary
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
/
Models, Genetic
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2009
Type:
Article
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