Rescue of chimeric rabies virus expressing green fluorescent protein / 病毒学报
Chinese Journal of Virology
;
(6): 401-403, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-334788
ABSTRACT
Green fluorescent protein (GFP) gene was inserted into the pseudogene (psi) region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene (psi) region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rabies virus
/
Recombinant Proteins
/
Cell Line
/
Green Fluorescent Proteins
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2008
Type:
Article
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