Immune response induced by human cytomegalovirus DNA vaccine / 病毒学报
Chinese Journal of Virology
;
(6): 320-321, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-334802
ABSTRACT
Human cytomegalovirus is an important pathogen which widely infects human. In this study, an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly, the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot. The maxiprepared plasmid pVAX1/gB680 + pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined. Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells, and could induce antibodies and cellular immune responses in BALB/c mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Chlorocebus aethiops
/
COS Cells
/
Vaccines, DNA
/
Cytomegalovirus Vaccines
/
Allergy and Immunology
/
Mice, Inbred BALB C
/
Antibodies, Viral
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2008
Type:
Article
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