Infection and latency of HSV-1 on rat bone marrow mesenchymal stem cells in vitro / 病毒学报
Chinese Journal of Virology
;
(6): 272-276, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-334811
ABSTRACT
To culture bone marrow mesenchymal stem cells (BMSCs) of rat in vitro and observe HSV-1 infection on BMSCs, BMSCs were separated from the bone marrow and identified by alizarin red staining and detection of ALP. The morphology of HSV-1 infected BMSCs and the CPE were observed. The total DNA was extracted from HSV-1 infected BMSCs and the desired specific gene fragment of 477bp of HSV-1 was amplified by PCR. Results showed that after BMSCs were induced by mineral-fluid for 14 days, the ALP level was increased and the nodule calcification was formed. The induced BMSCs were manifested to have the characteristics of osteoblasts. CPE couldn't be found in HSV-1 latently infected BMSCs but the 477bp gene fragment was still detectable. HSV-1 could establish latent infection in BMSCs after 7 passages. This study indicated that rat BMSCs could be induced to differentiate into osteoblasts in vitro, therefore they can be used as the seed cells for the tissue engineering. HSV-1 can infect rat BMSCs and develop the latent infection in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Bone Marrow Cells
/
Cell Differentiation
/
Polymerase Chain Reaction
/
Rats, Wistar
/
Virus Latency
/
Herpesvirus 1, Human
/
Cell Biology
/
Tissue Engineering
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2008
Type:
Article
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