Construction, expression and immunogenicity of eukaryotic vectors based on goat pox virus P32 gene / 病毒学报
Chinese Journal of Virology
;
(6): 133-137, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-334835
ABSTRACT
The full-length P32 gene and the truncated P32 gene (MP-32) were amplified from the recombinant plasmid pMD-P32 by polymerase chain reaction (PCR) and cloned into pcDNA3. 1(+) and pcDNA3.1-CpG respectively. The recombinant plasmids (pcDNA3.1-P32, pcDNA3.1-CpG-P32 and pcDNA3. 1-CpG-MP32) were transfected into BHK-21 cells by using lipofectin. The expressed P32 protein was confirmed by indirect immunofluorescence assay (IFA). The BALB/c mice were immunized with these recombinant plasmids by intramuscular injection. The specific antibodies aginst CPV were detected by ELISA kit weekly. The murine splenic T lymphocyte subgroups CD4+ and CD8+ were detected by flow cytometry. Results showed that the P32 protein was expressed successfully in vitro. After 2 weeks post im munization, the specific IgG antibodies against CPV were detected in the vaccinated mice. The percentage of CD4+ /CD8+ T cells was significantly higher than that of the control. In conclusion, these constructed eukaryotic vectors could induce humoral and celluar immune responses in mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Recombinant Proteins
/
Viral Vaccines
/
Vaccines, Synthetic
/
Cell Line
/
Viral Envelope Proteins
/
T-Lymphocyte Subsets
/
Capripoxvirus
/
CpG Islands
/
Allergy and Immunology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS