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Molecular characterization of cotton leaf Curl Multan virus and its satellite DNA that infects Hibiscus rosa-sinensis / 病毒学报
Chinese Journal of Virology ; (6): 64-68, 2008.
Article in Chinese | WPRIM | ID: wpr-334847
Responsible library: WPRO
ABSTRACT
Virus isolate G6 was obtained from Hibiscus rosa-sinensis showing yellow and leaf curl symptoms in Guangzhou, Guangdong Province. The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides encoding six potential ORFs. Comparison showed that G6 DNA-A had more than 89% sequence identify with all isolates of Cotton leaf curl Multan virus (CLCuMV) and shared the highest sequence identify (96.1%) with CLCuMV isolate 62. G6 DNA-A had 87.1%-89.8% sequence identity with those of CLCuRV isolates, while less than 87% identities with other begomoviruses. Phylogenetic analysis of G6 DNA-A and selected begomoviruses showed that G6 was most closely related to CLCuMV isolates, and they clustered together as a separate branch. Satellite DNA molecule (G6 DNAbeta) was found to be associated with G6 using the primers beta01 and beta02. G6 DNAbeta contains 1346 nucleotides, with a potential functional ORF (C1) in complementary sense DNA. Pairwise comparison indicated that G6 DNAbeta had the highest sequence identities with CLCuMV DNAbeta (92.1%) and CLCuRV DNAbeta (88.7%), but less than 80% sequence identities with other reported satellite DNA molecules. Phylogenetic analysis indicated that G6 DNAbeta was most closely related to CLCuMV DNAbeta and the two DNAbetas clustered together as a separate branch, and formed the main branch with DNAbeta of CLCuRV and MYVV-Y47. It is concluded that G6 infecting Hibiscus rosa-sinensis is an isolate of CLCuMV.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Virology / DNA, Satellite / DNA, Viral / Base Sequence / Chemistry / Classification / Geminiviridae / Gossypium / Hibiscus Language: Chinese Journal: Chinese Journal of Virology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Virology / DNA, Satellite / DNA, Viral / Base Sequence / Chemistry / Classification / Geminiviridae / Gossypium / Hibiscus Language: Chinese Journal: Chinese Journal of Virology Year: 2008 Type: Article