Generation of cell culture high-yield recombinant H3N2 subtype swine influenza vaccine candidate by reverse genetics / 病毒学报
Chinese Journal of Virology
;
(6): 471-476, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-334863
ABSTRACT
High-yield H3N2 subtype swine influenza virus for large-scale vaccine production in cell culture was generated by reverse genetics. The rescued H3N2 (rH3N2) candidate virus contained hemagglutinin (HA) and neuraminidase (NA) genes derived from a field isolate A/Swine/Henan/S4/01 (H3N2), PB2 gene from A/PR/8/34, and the other five internal genes from A/Goose/Dalian/3/01 (H9N2). The rH3N2 virus titer in MDCK cell culture were measured by hemagglutination assay and the maximum virus titre of 1512 hemagglutination unit was obtained after infection of MDCK cell for 60 h. The results of the present study indicated that rH3N2 virus was suitable for growth in MDCK cell culture and is feasible to be used for the production of cell grown influenza vaccine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Virus Cultivation
/
Hemagglutination Tests
/
Influenza Vaccines
/
Cell Line
/
Classification
/
Influenza A Virus, H3N2 Subtype
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2007
Type:
Article
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