Subcellular localization of APOBEC3G by confocal laser scanning microscope (CLSM) / 病毒学报
Chinese Journal of Virology
;
(6): 16-21, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-334916
ABSTRACT
Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) cDNA was amplified from total RNA prepared from nonpermissive H9 cells by RT-PCR. APOBEC3G cDNA is 1155nt long, encoding 384 amino acids. The APOBEC3G gene was then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-3G construct was then transfected into CD4+ HeLa cell to determine the expression and the subcellular localization of GFP-APOBEC3G fusion protein. Under CLSM the localization of the expressed GFP-APOBEC3G in the cytoplasm of CD4+ HeLa cells was observed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Repressor Proteins
/
Recombinant Fusion Proteins
/
HeLa Cells
/
Cell Line
/
DNA, Complementary
/
Microscopy, Confocal
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cytidine Deaminase
/
Cytoplasm
/
Green Fluorescent Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2007
Type:
Article
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