Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1230-1232, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-334952
ABSTRACT
<p><b>OBJECTIVE</b>To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.</p><p><b>METHODS</b>CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.</p><p><b>RESULTS</b>With the optimal molar ratio of CDTPA/Ab at 201, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.</p><p><b>CONCLUSION</b>(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Yttrium Radioisotopes
/
Chemistry
/
Leukocyte Common Antigens
/
Immunoconjugates
/
Radiopharmaceuticals
/
Pentetic Acid
/
Allergy and Immunology
/
Isotope Labeling
/
Anhydrides
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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