Progesterone-modulated proteins in human endometrial cancer cell line Ishikawa / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1110-1113, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-334984
ABSTRACT
<p><b>OBJECTIVE</b>To identify proteins associated with growth inhibitory effects of progesterone in Ishikawa endometrial adenocarcinoma cell line (Ishikawa).</p><p><b>METHODS</b>The total cellular proteins of the control and megestrol acetate (MA)-treated Ishikawa cells were separated by two-dimensional gel electrophoresis. After colloidal Coomassie G-250 staining and scanning, the gel images were analyzed by PDQuest software. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and Mascot software were used to identify the differentially expressed protein.</p><p><b>RESULTS AND CONCLUSIONS</b>Well-resolved and reproducible 2-dimensional patterns of both control and MA-treated Ishikawa cells were obtained. Forty-one proteins displayed differential expression after MA treatment, among which 23 were up-regulated and 18 down-regulated. Peptide mass fingerprints (PMF) by MALDI-TOF-MS analysis and search in NCBInr resulted in the identification of 8 proteins. The up-regulated proteins identified were catechol-O-methyltransferase (COMT), glutathione S-transferase, keratin 8, splicing factor 3a (subunit 3), chaperonin containing TCP1 (subunit 6A isoform a, CCT6A), RAB6A protein and AHA1. The down-regulated protein was peptidyl prolyl isomerase A (isoform 1). These findings can be helpful in further investigation of the molecular mechanism of progesterone.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Progesterone
/
Mass Spectrometry
/
Electrophoresis, Gel, Two-Dimensional
/
Adenocarcinoma
/
Proteins
/
Down-Regulation
/
Up-Regulation
/
Endometrial Neoplasms
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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