Bevacizumab (Avastin) inhibits lung cancer-induced pulmonary microvascular angiogenesis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of bevacizumab (Avastin) on pulmonary microvascular angiogenesis induced by lung cancer.</p><p><b>METHODS</b>The effects of Avastin on cell growth, cell cycle and apoptosis of lung microvascular endothelial cells were assayed by flow cytometry.</p><p><b>RESULTS</b>Avastin inhibited the growth of lung microvascular endothelial cells and induced increase of cell apoptosis index in a concentration-dependent manner. With the increment of Avastin concentration from 25 to 100 microg/ml, avascular area increased from 0.944-/+0.073 cm square to 5.189-/+0.192 cm square, and the cell apoptosis index increased from 32.5%-/+1.5% to 39.25-/+1.6%.</p><p><b>CONCLUSION</b>Avastin can inhibit the growth and induce apoptosis of lung microvascular endothelial cells in three-dimensional culture, possibly in association with cell cycle arrest in G(0/1) phase.</p>