Screening of short peptides binding to cell surface interleukin-2 receptor alpha chain / 南方医科大学学报
Journal of Southern Medical University
;
(12): 971-974, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-335017
ABSTRACT
<p><b>OBJECTIVE</b>To screen and characterize the short peptides which bind specifically to interleukin-2 (IL-2) receptor alpha chain (IL-2Ralpha) for acquisition of small antagonists for blocking the binding of IL-2 with IL-2Ralpha.</p><p><b>METHODS</b>12-mer phage displayed peptide library was screened with the target cells of MT-2 cells which expressed IL-2Ralpha at high levels. The binding phage clones were eluted by anti-IL-2Ralpha monoclonal antibody. After 3 rounds of screening, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences of the positive clones were deduced from the DNA sequences.</p><p><b>RESULTS</b>Seven positive clones were screened out of the 17 phage clones bound to MT-2 cells. The positive clone M15 could bind specifically to MT-2 cell and PHA-activated peripheral blood monouclear cells. Amino acid sequence analysis identified 6 sequences, all of which contained hydrophilic residues, and 5 of these 6 sequences included Tyr, Phe and Leu conservative residues.</p><p><b>CONCLUSION</b>The peptide sequences containing Tyr, Phe conservative residues identified in this study can bind to cell surface IL-2Ralpha.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Protein Binding
/
Enzyme-Linked Immunosorbent Assay
/
Cell Line, Transformed
/
Immunohistochemistry
/
T-Lymphocytes
/
Amino Acid Sequence
/
Interleukin-2
/
Receptors, Cell Surface
/
Peptide Library
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS