Antioxidative effect of folate-modified chitosan nanoparticles
Asian Pacific Journal of Tropical Biomedicine
;
(12): 29-38, 2011.
Article
in English
| WPRIM
| ID: wpr-335068
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the potency of carboxymethyl chitosan-2, 2' ethylenedioxy bis-ethylamine-folate (CMC-EDBE-FA) on tissue injury, antioxidant status and glutathione system in tissue mitochondria and serum against nicotine-induced oxidative stress in mice.</p><p><b>METHODS</b>CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2, 2' ethylenedioxy bis-ethylamine. Animals were divided into four groups, i.e., control, nicotine (1 mg/kg bw/day), CMC-EDBE-FA (1 mg/kg bw/day) and nicotine (1 mg/kg bw/day) and CMC-EDBE-FA (1 mg/kg bw/day) for 7 days. Levels of lipid peroxidation, oxidized glutathione level, antioxidant enzyme status and DNA damage were observed and compared.</p><p><b>RESULTS</b>The significantly increase of lipid peroxidation, oxidized glutathione levels and DNA damage was observed in nicotine treated group as compared with control group; those were significantly reduced in CMC-EDBE-FA supplemented group. Moreover, significantly reduced antioxidant status in nicotine treated group was effectively ameliorated by the supplementation of CMC-EDBE-FA. Only CMC-EDBE-FA treated groups showed no significant change as compared with control group; rather than it repairs the tissue damage of nicotine treated group.</p><p><b>CONCLUSIONS</b>These findings suggest that CMC-EDBE-FA is non-toxic and ameliorates nicotine-induced toxicity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Specificity
/
Oxidoreductases
/
Pharmacology
/
Chemistry
/
Chitosan
/
Toxicity
/
DNA Fragmentation
/
Nanoparticles
/
Folic Acid
/
Glutathione
Limits:
Animals
Language:
English
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2011
Type:
Article
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