Isolation and characterization of side population cells in human gastric cancer cell line BGC-823 / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 264-268, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-335299
ABSTRACT
<p><b>OBJECTIVE</b>Isolate and characterize the side population (SP) cells with potency of stem cells from human gastric carcinoma cell line BGC-823.</p><p><b>METHODS</b>SP and non-SP cells were sorted from BGC-823 cells by fluorescence-activated cell sorting (FACS) using Hoechst33342 staining. The tumorigenic ability of the SP cells was assessed by in vivo transplantation into non-obese diabetic/severe combined immunodeficiency mice.</p><p><b>RESULTS</b>SP cells were isolated from BGC-823 cells in a proportion of 0.9% to 2.1% with respect to the whole cell population. The colony formation assay showed that the colony formation rate of the SP cells was significantly higher than that of the non-SP cells (72.56% vs. 49.00%, P < 0.01). The drug sensitivity test showed that the SP cells showed stronger drug resistance to 5-Fu than the non-SP cells. The in vivo transplantation of SP cells in mice showed that the tumor weight was (0.176 ± 0.034) g, significantly higher than that of non-SP cells (0.045 ± 0.046) g (P < 0.01).</p><p><b>CONCLUSIONS</b>The results of this study indicate the existence of cancer stem-like SP cells in the human gastric cancer line BGC-823 cells. Further characterization of this SP cell population may provide new insights for diagnosis and treatment of gastric cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Transplantation
/
Mice, SCID
/
Mice, Inbred NOD
/
Colony-Forming Units Assay
/
Drug Resistance, Neoplasm
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2012
Type:
Article
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