New compound NL-608 (a nutlin analog) induces apoptosis in human breast cancer MCF-7 cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 100-103, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-335335
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of NL-608 (a nutlin analog) on apoptosis induction in human breast cancer MCF-7 cells in vitro, and investigate the relevant molecular mechanism.</p><p><b>METHODS</b>The effect of NL-608 on proliferation of MCF-7 cells was determined by MTT assay. The apoptosis in MCF-7 cells was determined by flow cytometry with annexin V-FITC and PI. The activity of caspase 3, caspase 8 and caspase 9 was determined with caspase activity assay kit and Western blot, and the proteins of Fas and FasL were determined by Western blot.</p><p><b>RESULTS</b>NL-608 showed a dose-dependent inhibitory effect on the proliferation of MCF-7 cells. It induced apoptosis in MCF-7 cells in a dose-dependent manner. The activity of caspase 3 and caspase 8 in MCF-7 cells was increased with the increasing concentration of NL-608, but caspase 9 had no changes. The proteins of Fas and FasL were increased in a dose-dependent manner.</p><p><b>CONCLUSION</b>NL-608 induces apoptosis in MCF-7 cells in vitro through inducing caspase 3 activity and death receptor-mediated signal pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Piperazines
/
Apoptosis
/
Fas Receptor
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Caspase 3
/
Caspase 8
/
Caspase 9
/
Fas Ligand Protein
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2012
Type:
Article
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