Association between H-ras and L-myc gene polymorphisms and susceptibility to colorectal cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 15-20, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-335353
ABSTRACT
<p><b>OBJECTIVE</b>To explore the association between the polymorphisms of oncogenes H-ras and L-myc and colorectal cancer risk, and the interaction of those genes.</p><p><b>METHODS</b>The genotypes of H-ras and L-myc genes were determined by polymerase chain reaction-based restriction fragment length polymorphism analysis. Stratified analysis and logistic model were used to detect the gene-gene interaction. The gene-gene interaction was validated by multifactor dimensionality reduction (MDR) analysis.</p><p><b>RESULTS</b>The single SNP model showed that the polymorphisms of H-ras and L-myc genes were not significantly related with colorectal cancer risk (P > 0.05). Stratified analysis revealed that among the L-myc LS + SS genotype carriers, those with H-ras TC + CC genotype showed significantly increased risk of rectal cancer than those with TT genotype (OR = 1.81, P = 0.005). The positive interaction between L-myc and H-ras was detected by logistic regression model. The OR of the interaction effect was 2.74 (P = 0.024). This result was confirmed in the MDR model, with 54.83% testing balanced accuracy and 10/10 cross-validation consistency, and the model was still significant after the 1000 times permutation test (P = 0.001).</p><p><b>CONCLUSION</b>Our findings suggest that the polymorphism of H-ras and L-myc genes is not related to colorectal cancer risk, but there is a synergy between H-ras and L-myc polymorphisms in the development of rectal cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rectal Neoplasms
/
Polymorphism, Restriction Fragment Length
/
Colorectal Neoplasms
/
Logistic Models
/
Polymerase Chain Reaction
/
Risk
/
Surveys and Questionnaires
/
Genes, myc
/
Genes, ras
/
Colonic Neoplasms
Type of study:
Etiology study
/
Prognostic study
/
Risk factors
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2012
Type:
Article
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