Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 87-92, 2009.
Article
in English
| WPRIM
| ID: wpr-335396
ABSTRACT
The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice. Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA). After 72-h culture, Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached. Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression. Ginsenosides (1.0 approximately10 microg/ml) significantly stimulated proliferation of spermatogonia. Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10(-8) to 10(-7) mol/L and the PKC inhibitor H(7) inhibited this effect. Likewise, ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H(7). These results indicate that the proliferating effect of ginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Spermatogonia
/
Protein Kinase C
/
Proliferating Cell Nuclear Antigen
/
Cell Biology
/
Ginsenosides
/
Cell Proliferation
/
Enzyme Activation
/
Mice, Inbred ICR
Limits:
Animals
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2009
Type:
Article
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