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Removal of lipopolysaccharides from anti-complementary crude polysaccharides of Houttuynia Herba / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3398-3402, 2017.
Article in Chinese | WPRIM | ID: wpr-335842
ABSTRACT
An Affi-Prep Polymyxin column was combined with a Phenyl Sepharose column and a Sephacryl S-300 column, respectively, to remove the lipopolysaccharides(LPS) in the anti-complementary crude polysaccharides of Houttuynia Herba. The contents of LPS in the polysaccharides were determined by chromogenic tachypleus amebocyte lysate(TAL)method during the procedure of purifying. The anti-complementary activities of the polysaccharides were also compared before and after the removal of LPS. Less remanent LPS was detected after purified using Penyl Sepharose combined with polymyxin column, with the clearance rate of 42.85%. All the columns had no effect on the anti-complementary activity of the polysaccharides. Penyl Sepharose combined with polymyxin column would be sound for LPS removal of the anti-complementary polysaccharides without reducing their bioactivity.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2017 Type: Article