Expression of Kir2.1, SCN5a and SCN1b channel genes in mouse cardiomyocytes with various electric properties: patch clamp combined with single cell RT-PCR study / 生理学报
Acta Physiologica Sinica
;
(6): 82-86, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-335938
ABSTRACT
This study is to explore a new method of investigating molecular basis for electrophysiological properties of early fetal cardiomyocytes. Single embryonic cardiomyocytes of mouse early developmental heart (E10.5) were obtained by a collagenase B digestion approach. After recording spontaneous action potential using whole cell patch clamp technique, the single cell was picked by a glass micropipette, followed by a standard RT-PCR to explore the expression levels of several ion channel genes. Three phenotypes of cardiomyocytes were demonstrated with distinct properties ventricular-like, atrial-like, and pacemaker-like action potentials. Ventricular-like and atrial-like cells were characterized with much negative maximum diastolic potential (MDP) and a higher V(max) (maximum velocity of depolarization) compared to pacemaker-like cells. MDP of ventricular-like cells was the most negative. In parallel, stronger expression of SCN5a, SCN1b and Kir2.1 were observed in ventricular-like and atrial-like cells compared to that of pacemaker-like cells, where Kir2.1 in ventricular-like cells was the most abundant. Cardiomyocytes with distinct electrophysiological properties had distinct gene expression pattern. Single cell RT-PCR combined with patch clamp technique could serve as a precise detector to analyze the molecular basis of the special electrophysiological characteristics of cardiomyocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Patch-Clamp Techniques
/
Potassium Channels, Inwardly Rectifying
/
Myocytes, Cardiac
/
Electrophysiological Phenomena
/
Fetus
/
Real-Time Polymerase Chain Reaction
/
Voltage-Gated Sodium Channel beta-1 Subunit
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2012
Type:
Article
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