Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy / 生理学报
Acta Physiologica Sinica
;
(6): 267-271, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-335991
ABSTRACT
The present study aims to investigate the effects of soluble endoglin (sEng) on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by trypsin digestion method, and were incubated with cell culture medium without (control group) and with 10 μg/L sEng (sEng group), respectively for 24 h. The invasive ability was determined by transwell invasion assay, and expressions of MMP-2, MMP-9 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results showed that the invasive ability of cytotrophoblasts in sEng group was lower than that in control group (P < 0.05). Compared with control group, the expressions of MMP-2 and MMP-9 mRNA and protein of cytotrophoblasts were significantly lower (P < 0.05). In conclusion, sEng may participate in the genesis of preeclampsia by affecting the invasive ability of cytotrophoblasts through regulation of the expression of MMP-2 and MMP-9.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Placentation
/
Pre-Eclampsia
/
Pregnancy Trimester, First
/
Trophoblasts
/
RNA, Messenger
/
Antigens, CD
/
Cell Movement
/
Cells, Cultured
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2011
Type:
Article
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