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Involvement of cAMP-PKA pathway in group Ⅱ metabotropic glutamate receptors-mediated regulation of respiratory rhythm from neonatal rat brainstem slice / 生理学报
Acta Physiologica Sinica ; (6): 233-237, 2011.
Article in Chinese | WPRIM | ID: wpr-335996
ABSTRACT
The study aims to identify the role of cAMP-PKA pathway in the group Ⅱ metabotropic glutamate receptors (mGluRs)-mediated regulation of respiratory rhythm from the brainstem slice. Neonatal (aged 0-3 d) Sprague-Dawley rats of either sex were used. The brainstem slice containing the medial region of the nucleus retrofacialis (mNRF) and the hypoglossal nerve rootlets was prepared, and the surgical procedure was performed in the modified Kreb's solution (MKS) with continuous carbogen (95% O2 and 5% CO2) bubbling, and ended in 3 min. Respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve rootlets was recorded by suction electrode. Eighteen brainstem slice preparations were divided into 3 groups. In group 1, group Ⅱ mGluRs specific antagonist (2S)-α-ethylglutamic acid (EGLU) was added into the perfusion solution for 10 min. In group 2, after application of Forskolin for 10 min, washout with MKS, the slice was perfused with Rp-cyclic 3', 5'-hydrogen phosphorothioate adenosine triethylammonium salt (Rp-cAMPS) alone for another 10 min. In group 3, after application of Rp-cAMPS for 10 min, additional EGLU was added into the perfusion for another 10 min. The results showed EGLU shortened respiratory cycle (RC), but the changes of integral amplitude (IA) and inspiratory time (TI) were not statistically significant. Forskolin induced significant decreases in RC, and increased TI, IA. Rp-cAMPS could make the opposite effect compared with the changes of RRDA with Forskolin. The effect of EGLU on the RRDA was inhibited after blocking the cAMP-PKA pathway. Taken together, cAMP-PKA pathway may play an important role in the group Ⅱ mGluRs-mediated regulation of RRDA in the brainstem slice of neonatal rats.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Respiration / In Vitro Techniques / Brain Stem / Signal Transduction / Rats, Sprague-Dawley / Receptors, Metabotropic Glutamate / Cyclic AMP-Dependent Protein Kinases / Animals, Newborn / Metabolism Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Respiration / In Vitro Techniques / Brain Stem / Signal Transduction / Rats, Sprague-Dawley / Receptors, Metabotropic Glutamate / Cyclic AMP-Dependent Protein Kinases / Animals, Newborn / Metabolism Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2011 Type: Article