MTT assay for detecting osteosarcoma cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1899-1901, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-336055
ABSTRACT
<p><b>OBJECTIVE</b>To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.</p><p><b>METHODS</b>The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.</p><p><b>RESULTS</b>MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.</p><p><b>CONCLUSION</b>MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
General Surgery
/
Tetrazolium Salts
/
Thiazoles
/
Bone Neoplasms
/
Tumor Cells, Cultured
/
Antineoplastic Combined Chemotherapy Protocols
/
Osteosarcoma
/
Apoptosis
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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