Expression of Aurora-B in non-small cell lung cancer and its clinical significance / 南方医科大学学报
Journal of Southern Medical University
; (12): 1853-1856, 2009.
Article
in Zh
| WPRIM
| ID: wpr-336066
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of Aurora-B in non-small cell lung cancer (NSCLC) tissues and NSCLC cell lines.</p><p><b>METHOD</b>Aurora-B expression was examined using immunohistochemical SP method in 91 stage I and 69 stage II-III NSCLC tissues and 40 adjacent tissues. The mRNA and protein expressions of Aurora-B in NSCLC cell lines (A549, H460 and H1299) were examined by RT-PCR and Western blotting, respectively.</p><p><b>RESULTS</b>The protein expression of Aurora-B was detected in 77.7% (94/121) of the tumor tissues and 9.8% (4/41) of the adjacent tissues, showing a significant difference between them (P<0.01). The positivity rate of Aurora-B protein was not related with the gender and age of NSCLC patients, but with lymph node metastasis, differentiation and histological type of NSCLC (P<0.05). Aurora-B was expressed in all the NSCLC cell lines (A549, H460 and H1299) at both mRNA and protein levels. A549 cells showed the highest expression of Aurora-B.</p><p><b>CONCLUSION</b>Aurora-B protein is highly expressed in NSCLC tissues and cell lines, and may play a crucial role in the invasion, metastasis and development of NSCLC. The mRNA and protein expression levels of Aurora-B differ significantly between different NSCLC cell lines.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
RNA, Messenger
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Tumor Cells, Cultured
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Protein Serine-Threonine Kinases
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Carcinoma, Non-Small-Cell Lung
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Aurora Kinases
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Aurora Kinase B
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Genetics
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Lung Neoplasms
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Lymphatic Metastasis
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article