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Expression of oxyntomodulin in bifidobacteria and effect of oxyntomodulin-transformed bifidobacteria on the body weight of obese mice / 南方医科大学学报
Journal of Southern Medical University ; (12): 1796-1798, 2009.
Article in Chinese | WPRIM | ID: wpr-336083
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of pBBADs-OXM-transformed bifidobacteria on the body weight of obese mice.</p><p><b>METHODS</b>B. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed.</p><p><b>RESULTS</b>OXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group (P<0.05).</p><p><b>CONCLUSION</b>Administration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Appetite Depressants / Bifidobacterium / Body Weight / Recombinant Proteins / Random Allocation / Administration, Oral / Electroporation / Drug Therapy / Escherichia coli / Oxyntomodulin Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Appetite Depressants / Bifidobacterium / Body Weight / Recombinant Proteins / Random Allocation / Administration, Oral / Electroporation / Drug Therapy / Escherichia coli / Oxyntomodulin Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2009 Type: Article