Biocompatibility of a novel cavernous nickel-titanium alloy with rat bone marrow stromal cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1787-1789, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-336086
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biocompatibility of a novel cavernous nickel-titanium alloy with rat bone marrow stromal cells (BMSCs) in vitro.</p><p><b>METHODS</b>Rat BMSCs were cultured on the surface of compact, microporous and macroporous nickel-titanium alloys, and the cell proliferation on day 3 during the culture was assessed using MTT assay. On day 7 of the cell culture, the cells were labeled with Hoechst33342 for cell counting under a fluorescence microscope. Scanning electron microscopy (SEM) was performed on day 7 of cell culture to observe the morphological changes of the cells.</p><p><b>RESULTS</b>The cell proliferation rate and cell numbers differed significantly between the cavernous alloy groups and the compact alloy group (P<0.05), but similar between the former two groups (P>0.05). SEM showed that compared with the compact alloy, microporous and macroporous nickel-titanium alloys had better biocompatibility with the BMSCs, and the cells on the surface of the cavernous alloys had normal cell morphology.</p><p><b>CONCLUSION</b>Cavernous nickel-titanium alloy has good biocompatibility and can promote the adhesion, aggregation and proliferation of rat BMSCs in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Titanium
/
Biocompatible Materials
/
Materials Testing
/
Bone Marrow Cells
/
Cell Adhesion
/
Cells, Cultured
/
Stromal Cells
/
Rats, Sprague-Dawley
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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