Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.</p><p><b>METHODS</b>Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.</p><p><b>RESULTS</b>After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05).</p><p><b>CONCLUSION</b>Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.</p>