Expression of porcine interferon-gamma and its safe antiviral assay / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 439-447, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-336208
ABSTRACT
In order to ensure the biosafety of the IFN-gamma antiviral activity assay, we used a replication-deficient VSV carrying GFP as an interferon sensitive indicator virus (VSVdeltaG*G). The antiviral activities of porcine IFN-gamma expressed in Escherichia coli and in baculovirus on MDBK cells were assessed. The results showed that the antiviral activity of porcine IFN-gamma expressed in baculovirus could reach 10(5) IU/mL, while the porcine IFN-gamma expressed in E. coli showed some antiviral activity (32 IU/mL) after refolding. The results of the VSVdeltaG*G-based antiviral assay were almost identical to that of the VSV*GFP-based assay, suggesting it is highly feasible to use VSVdeltaG*G as a substitute for VSV*GFP, making assays for IFN-gamma antiviral activity safer and more accurate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pharmacology
/
Physiology
/
Swine
/
Recombinant Proteins
/
Baculoviridae
/
Interferon-gamma
/
Vesiculovirus
/
Green Fluorescent Proteins
/
Escherichia coli
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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