Expression and purification of fusion protein CTP-SOD in Pichia pastoris and antioxidant capacity analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 324-329, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-336224
ABSTRACT
Cytoplasmic transduction peptide (CTP) is a newly designed transduction peptide, by which special molecules can be carried out and localized into cytoplasmic compartment. Superoxide dismutase (SOD) is a protein that is difficult to go into cytoplasm. In this study, CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris. HeLa cells pretreated with CTP-SOD showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-SOD could cross the cell membrane more efficiently and protect cells from oxidative stress.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Pichia
/
Superoxide Dismutase
/
Recombinant Fusion Proteins
/
HeLa Cells
/
Signal Transduction
/
Oxidative Stress
/
Cytoplasm
/
Intracellular Signaling Peptides and Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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