Development of a fed-batch process for TNFR-fc producing GS-CHO cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 216-222, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-336239
ABSTRACT
TNFR-Fc is an important fusion protein that has great potential in therapeutic and diagnostic applications. We developed an efficient fed-batch process for GS-CHO cells to produce TNFR-Fc. The rationale of this fed-batch process relies on the supply of sufficient nutrients to meet the requirements of cell metabolism. The optimal feed medium was designed through ration design. A metabolically responsive feeding strategy was designed and dynamically adjusted based on the residual glucose concentration determined off-line. In this process, the maximal viable cell density and antibody concentration reached above 9.4x10(6) cells/mL and 207 mg/L, respectively. Compared with the batch process, the newly developed fed-batch process increased the cell yield by 3.4 fold and the final antibody concentration by 3 fold. This fed-batch process would therefore facilitate the production of therapeutic antibody by GS-CHO cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Immunoglobulin G
/
Cricetulus
/
CHO Cells
/
Receptors, Tumor Necrosis Factor
/
Cell Culture Techniques
/
Culture Media
/
Etanercept
/
Genetics
/
Glucose
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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