Metabolic characteristics and kinetic model of recombinant CHO cells in serum-free suspension batch culture / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 85-92, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-336257
ABSTRACT
By using the cell density, cell viability, Pro-UK activity, specific consumption rate of glucose (q(glc)), specific production rate of lactate (q(lac)), yield of lactate to glucose (Y(lac/glc)) and as the evaluation indexes, the growth and metabolism characteristics of pro-urokinase (Pro-UK) expressing CHO cells in serum-free suspension batch culture were examined and compared to those in serum-containing suspension batch culture. We observed hardly differences in growth and metabolism characteristics between the CHO cell populations grown in serum-free suspension batch culture and serum-containing suspension batch culture. The optimal mathematical model parameters for the CHO cells grown in suspension batch culture were obtained by non-linear programming of data representing the growth, substrate consumption and product formation of the CHO cells during logarithmic growth phase using MATLAB software, and the kinetic model of the cell growth and metabolism in serum-free culture were established.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Kinetics
/
Urokinase-Type Plasminogen Activator
/
Cricetulus
/
Culture Media, Serum-Free
/
CHO Cells
/
Bioreactors
/
Culture Techniques
/
Genetics
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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