Cytocompatibility of two porous bioactive glass-ceramic in vitro / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare the cytocompatibility of two kinds porous bioactive glass-ceramic made by same raw materials.</p><p><b>METHODS</b>Apatite/wollastonite bioactive glass-ceramic (4006) were prepared by sol-gel method, and bioactive glass (45S5) were prepared by melting method. Bone marrow stromal cells (BMSCs) were cultivated, differentiated and proliferated into osteoblasts, from a rabbit's marrow in the differentiatiofn culture medium with active function. The viability of BMSCs cultivated with extraction of these two kinds of biomaterial, which could represent the cytotoxicity effect of 4006 and 45S5 against BMSCs, was evaluated by the MTp assay. BMSCs were seeded and cocultivated with two kinds of biomaterial scaffolds respectively in vitro. The proliferation and biological properties of cells adhered to scaffolds were observed by inverted phase contrast microscope, scanning electron microscope (SEM), and environmental scanning electron microscope (ESEM), and a suitable cell amount for seeding on the scaffold was searched.</p><p><b>RESULTS</b>There was no difference on the viability of BMSCs only cultured for one day by complete extract of 4006 and culture medium (P>0.05), but there was significant difference between them when the cells had been cultured for 3 days(P<0.01). The extract of 45S5 had significantly higher cytotoxicity than extract of culture medium (P<0.01). The BMSCs adhered, spread, and proliferated throughout the pores of the scaffold 4006, and the amount of cells adhered to 4006 was more than to 45S5. The adhered cells to 4006 increased with the rising amount of cells seeded. And 2 x 10(7) cells.mL-1 suspension resulted inthe highest cell adherence during the comparative cells adherence test.</p><p><b>CONCLUSION</b>Apatite/woolastonite bioac tive glass-ceramic has good bioactivity and cytocompatibility. Therefore, it may have the potential to be a new cell vehicle for bone tissue engineering. And the suitable seeding cell amount of apatite/wollastonite bioactive glass-ceramic should be 2x10(7) cells.mL-1 or even more than that.</p>