Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 91-95, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-336385
ABSTRACT
<p><b>OBJECTIVE</b>Take human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.</p><p><b>METHODS</b>Train a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.</p><p><b>RESULTS</b>MTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.</p><p><b>CONCLUSION</b>HMGN2 can promote apoptosis of oral squamous cell carcinoma cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
In Vitro Techniques
/
Recombinant Proteins
/
Mouth Neoplasms
/
High Mobility Group Proteins
/
Carcinoma, Squamous Cell
/
Apoptosis
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2013
Type:
Article
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