Research of the synergetic action of fluid shear stress and 17-beta estradiol on the proliferation of MC3T3-E1 cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the suitable level and action time of 17-beta estradiol and fluid shear stress (FSS) and their combined effect on the proliferation of rat osteoblasts in vitro.</p><p><b>METHODS</b>MC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-beta estradiol and FSS values were applied respectively on MC3T3-E1, the suitable level of 17-beta estradiol and FSS were selected through MTT and alkaline phosphatase (ALP). Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity.</p><p><b>RESULTS</b>Seventeen-beta estradiol(10(-8) mol x L(-1) for 5 d and 12 x 10(-5) N FSS for 60 min exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group.</p><p><b>CONCLUSION</b>Both 17-beta estradiol and FSS have a suitable threshold in promoting proliferation of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-beta estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.</p>