Identification of proteins that interact with murine cytomegalovirus early protein M112-113 in brain / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3532-3536, 2011.
Article
in English
| WPRIM
| ID: wpr-336532
ABSTRACT
<p><b>BACKGROUND</b>Murine cytomegalovirus (MCMV) early protein M112-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis. To investigate the biological function of M112-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus (CMV), a screening for proteins interacting with M112-113 was performed by a yeast two-hybrid system.</p><p><b>METHODS</b>Bait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast. After confirmation of the expression of MCMV M112-113 in yeast, the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M112-113. Interactions between M112-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.</p><p><b>RESULTS</b>Two proteins interacting with M112-113 were identified, including metastasis-associated 1 (MTA1) and zinc finger, CCHC domain containing 18 (ZCCHC18). M112-113 protein could interact with MTA1 or ZCCHC18 in yeast and mammalian cells.</p><p><b>CONCLUSION</b>The interactions of M112-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Protein Binding
/
Viral Proteins
/
Brain
/
Cell Line
/
Muromegalovirus
/
Two-Hybrid System Techniques
/
Immunoprecipitation
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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