Effect of chronic lead exposure on expression of autophagy-associated proteins in rat hippocampus / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 402-409, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-336777
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of chronic lead exposure on expression of autophagy-associated proteins in rat hippocampus.</p><p><b>METHODS</b>SD rats were randomly divided into three groups control group was given distilled water, lead-exposed groups were given 0.5 g/L (low-dose) or 2.0 g/L(high-dose) lead acetate solution in drinking water. The rat pups started to drink the lead content water until 60 d maturity. The lead contents in blood and brain samples were analyzed by graphite furnace atomic absorption spectrophotometry. The expressions of Beclin 1, LC3, LAMP2 and cathepsin B proteins were detected by Western blot and immunohistochemistry.</p><p><b>RESULTS</b>Compared with control group, the contents of lead were significantly higher in blood and hippocampus samples in chronic lead-exposed rats (P<0.01). Western blot showed that the expression of Beclin 1 and LC3-II/LC3-I increased significantly in high dose lead-exposed group compared with control group (P<0.05 or P<0.001). The confocal laser immunostaining results demonstrated that increased immunofluorescence staining of cathepsin B in hippocampal neurons compared with control animals.</p><p><b>CONCLUSION</b>The disturbance of autophagy-lysosome signaling molecules might be partially contribute to neurotoxicity of chronic lead exposure.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Autophagy
/
Cathepsin B
/
Signal Transduction
/
Chronic Disease
/
Rats, Sprague-Dawley
/
Disease Models, Animal
/
Apoptosis Regulatory Proteins
/
Lysosomal-Associated Membrane Protein 2
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2012
Type:
Article
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