Isolation of rabbit aqueous humor-derived exosomes and their immunosuppression function / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 315-319, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-336790
ABSTRACT
<p><b>OBJECTIVE</b>To isolate exosomes from rabbit aqueous humor and to investigate their immunosuppression function.</p><p><b>METHODS</b>Aqueous humor was collected from 40 New Zealand rabbits and exosomes were isolated by fractional separation and ultracentrifugation methods; the morphology was studied with electron microscopy. The immunosuppressive-related proteins of exosomes were detected with Western blotting; their inhibitory effect on ConA-induced proliferation of T lymphocyte was estimation with CCK-8 cells proliferation assay.</p><p><b>RESULTS</b>Eight milliliters of aqueous humor were collected from 40 New Zealand rabbits and 200 μg exosomes was yielded. Under electron microscope, the exosomes had typical structure of lipid bi-layer with a diameter of 50-100 nm. The results of Western blotting showed that these exosomes expressed Hsp70, CD9 and Alix but not Grp94, presenting a typical exosomes protein profile. Moreover, exosomes expressed high level of TGF-β and significantly inhibited the proliferation of T lymphocytes.</p><p><b>CONCLUSION</b>Immunosuppressive exosomes can be isolated from rabbit aqueous humor, which may be involved in immunotolerance of the eye.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aqueous Humor
/
T-Lymphocytes
/
Cells, Cultured
/
Allergy and Immunology
/
Exosomes
/
Immune Tolerance
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2012
Type:
Article
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