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Influence of GLP-1 on oxidative stress injury in non-alcoholic fatty liver disease rats / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 757-762, 2014.
Article in Chinese | WPRIM | ID: wpr-337104
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of glucagon-like peptide-1 (GLP-1) on liver oxidative stress injury using a rat model of non-alcoholic fatty liver disease.</p><p><b>METHODS</b>Sixty male Sprague-Dawley rats were fed 12 weeks of either a diet of normal chow (NC), for use as controls (n =15) or high-fat chow (HF), for use as models (n =45).The NC rats were administered normal saline, while the HF rats were treated with either normal saline (NS), for use as untreated model controls (n =10), low-dose GLP-1 (LG, 50 mutg/kg; n =10), mid-dose GLP-1 (MG, 100 mutg/kg; n =10), or high-dose GLP-1 (HG, 200 mug/kg; n =10); all treatments lasted for 4 weeks.The rats' weight, levels of serum biochemical markers (triglycerides, total cholesterol, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol, alanine arninotransferase, and aspartate aminotransferase), levels of superoxide dismutase (SOD) and malondialdehyde (MDA), and expression of CYP2E1 mRNA and protein in liver homogenates were measured.The F test, t-test, least significant difference test and Dunnett's T3 test were used for statistical analyses.</p><p><b>RESULTS</b>Compared with the NC group, the rars in the NS group showed significantly lower SOD (165.81 ± 11.64 vs.192.89 ± 16.53 U/mg, P < 0.05), significantly higher MDA (7.30 ± 1.79 vs.3.10 ± 1.30 nmol/ mg, P < 0.05), and significantly higher expressions of CYP2E1 mRNA and protein (both P < 0.05).After GLP1 treatment, the rats in the LG, MG and HG groups showed increased levels of SOD (compared to the NS group; 171.44 ± 9.80 vs.177.66 ± 14.77 vs.186.17 ± 15.43 U/mg; only the HG group had P < 0.05), significantly decreased levels of MDA (compared to the NS group; 5.16 ± 1.45 vs.4.08 ± 1.22 vs.3.31 ± 1.14 nmol/mg; all P < 0.05], and decreased levels of CYP2E1 mRNA and protein expressions (CYP2E1 mRNAonly the HG group had P < 0.05; CYP2E1 protein both the MG and HG groups had P < 0.05).</p><p><b>CONCLUSION</b>GLP-1 treatment can improve oxidative stress injury, suggesting its potential as a therapeutic agent for non-alcoholic fatty liver disease.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Aspartate Aminotransferases / Superoxide Dismutase / Triglycerides / Rats, Sprague-Dawley / Oxidative Stress / Cytochrome P-450 CYP2E1 / Glucagon-Like Peptide 1 / Non-alcoholic Fatty Liver Disease / Malondialdehyde / Metabolism Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Aspartate Aminotransferases / Superoxide Dismutase / Triglycerides / Rats, Sprague-Dawley / Oxidative Stress / Cytochrome P-450 CYP2E1 / Glucagon-Like Peptide 1 / Non-alcoholic Fatty Liver Disease / Malondialdehyde / Metabolism Limits: Animals Language: Chinese Journal: Chinese Journal of Hepatology Year: 2014 Type: Article