Cloning, screening and sequence analysis of the major allergens of Psilgramma menephorn / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1145-1148, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-337310
ABSTRACT
<p><b>OBJECTIVE</b>To identify and isolate the genes encoding the allergens of Psilgramma menephorn by screening the cDNA expression library.</p><p><b>METHODS</b>The cDNA expression library of Psilgramma menephorn was constructed in lambdaZAPIIphage, and the library was screened using the sera from the patients allergic to Psilgramma menephorn and those from the rabbits immunized with Psilgramma menephorn extracts. The positive clones were subcloned into pBluescript plas, and the cDNA in the positive clones were amplified with PCR and sequenced.</p><p><b>RESULTS AND CONCLUSION</b>Five positive clones were obtained by immunological screening of 5 x 10(4) recombinants. Sequence analysis showed that the positive clones contained the new genes of Psilgramma menephorn allergens. This success in isolating these genes may facilitate the development of specific immunotherapy against Psilgramma menephorn allergy and further research of allergic diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Allergens
/
Gene Library
/
Immunization
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Genes, Insect
/
Electrophoresis, Polyacrylamide Gel
/
Allergy and Immunology
/
Genetics
/
Lepidoptera
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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